A current research project uses microfluidic devices to control stem cell differentiation. With these techniques, we are fabricating extracellular environments that mimic the in vivo environment. In one model system, we are investigating conditions for directing the differentiation of intestinal stem cells. We created spatial patterns of extracellular matrix proteins, and have successfully demonstrated our ability to control cell proliferation and differentiation in these engineered environments. In this research program, we are defining the complex combinations of stimuli that direct stem cell differentiation into functioning tissue.

A second program centers on neural regeneration. We are using similar strategies to uncover design rules for directing neuron growth, controlling cell polarity and synapse formation, and directing neural stem cell differentiation. This collaborative effort involves protein engineering, microfabrication, and neuroscience.