Services

The HTSF assists researchers in developing, optimizing and carrying out high-throughput screens of its chemical libraries.

Assay Development and Optimization
The HTSF can help develop assays using absorbance, fluorescence or luminescence and provide access to plate readers. The facility can also work with you to make your primary assay more suitable for high throughput screening and save reagent cost.
Potential targets for screens include enzymes, signal transduction pathways, gene regulatory proteins, micro RNAs and other macromolecules important in cell growth differentiation and signaling.

Provide Compound Libraries
We have chemical libraries containing approximately 175,000 small molecules in ~550 microtiter plates, which are easy to assay 96- and 384- well plate formats. A small subset of plates or the entire library can be screened in your assay.
You are welcome to donate compounds to our chemical collections.

Worry-free Screening
The facility can carry out the whole screening for you. You only need to provide us your protein/DNA/cell and protocol. We will perform the screening and send the data back to you. The facility has robotic equipment for protein-based and cell-based assays using 96 or 384 well microplates. Optical readouts include absorbance, fluorescence intensity, fluorescence polarization, FRET, time-resolved fluorescence and luminescence.

Equipment Resources
The HTSF provide the equipment resources that your laboratory personnel can use to develop assays or carry out the screen. The facility has sophisticated robotic liquid handling equipments which can dispense nanoliter and microliter volumes into 96 and 384 well plates. The plate readers are all automated for screening stacks of microplates.

Data Analyze and Follow-up Assays
Assist with data analyze can be done upon request. We can also help with hit validation and perform further testing of your macromolecule target and compounds such as dose-response curves, kinetic measurement, toxicity assays etc.

Cell Culture
With its separate cell culture room, the facility can assist with cell-based screens and cell-based follow up assays. You can also use our cell culture space if you want to perform cell-base experiments but do not have cell culture area in your lab.