Chad M. Rienstra
principal investigator
chemistry, biochemistry & biophysics
Professor Rienstra received his B.A. degree in chemistry from Macalester College (St. Paul, MN) in 1993 and his Ph.D. at the Massachusetts Institute of Technology in 1999, where he was a Howard Hughes Medical Institute Predoctoral Fellow. He was a National Institutes of Health postdoctoral fellow at Columbia University and joined the faculty at Illinois in 2002. His research interests are in solid-state nuclear magnetic resonance (SSNMR), including the development of new pulse sequence methodology and instrumentation, and application to studies of protein structure and dynamics. Disease-relevant systems under study include the synuclein family of proteins associated with Parkinson's disease, apolipoproteins involved with reverse cholesterol transfer, and cytochrome P450 monooxygenases responsible for drug metabolism.Please follow the research links to the left to find more information about these and other projects in the group.
Honors and Awards
- I.C. Gunsalus Scholar, 2008-2009
- Member, Incomplete List of Teachers Ranked as Excellent, Fall 2007
- Member, Incomplete List of Teachers Ranked as Excellent, Spring 2007
- Sloan Fellowship, 2007
- Keck Foundation Research Excellence Award, 2006
- Excellence in Teaching Award, School of Chemical Sciences, 2005
- Member, Incomplete List of Teachers Ranked as Excellent, Spring 2005
- National Center for Supercomputing Applications Fellow, 2005-2006
- Cottrell Scholars Award, Research Corporation, 2005-2007
- Beckman Fellow, UIUC Center for Advanced Study, 2005-2006
- Analytical Chemistry Award, Eli Lilly, 2004-2006
- CAREER Award, National Science Foundation, 2003-2008
- Research Innovation Award, Research Corporation, 2003-2005
- Petroleum Research Fund Award, American Chemical Society, 2003-2005
- National Institutes of Health Postdoctoral Fellowship, 2000-2002
- Howard Hughes Medical Institute Predoctoral Fellowship, 1993-1998
key publications (pubmed) (full list) (scopus)
methods
"High-performance solvent suppression for proton-detected solid-state NMR", Zhou, D. H.; Rienstra, C. M. J. Magn. Reson. 2008, in press. (Published online, February 1, 2008).
“Solid-state protein structure determination with proton-detected triple resonance 3D magic-angle spinning NMR spectroscopy”, Zhou, D. H.; Shea, J. J.; Nieuwkoop, A. J.; Franks, W. T.; Wylie, B. J.; Mullen, C.; Sandoz, D.; Rienstra, C. M., Angew. Chem. 2007, 46, 8380-8383. (Published online October 1, 2007).
“Proton-detected solid-state NMR spectroscopy of fully protonated proteins at 40 kHz magic-angle spinning”, Zhou, D. H.; Shah, G.; Cormos, M.; Mullen, C.; Sandoz, D.; Rienstra, C. M., J. Am. Chem. Soc. 2007, 129, 11791-11801. (Published online August 29, 2007).
“Backbone assignments in solid-state proteins using J-based 3D heteronuclear correlation spectroscopy”, Chen, L.; Kaiser, J. Michael; Polenova, T.; Yang, J.; Rienstra, C. M.; Mueller, L. J., J. Am. Chem. Soc. (Communication) 2007, 129, 10650-10651. (Published online, August, 11, 2007).
“Four-dimensional heteronuclear correlation experiments for chemical shift assignment of solid proteins”, Franks, W. T.; Kloepper, K. D.; Wylie, B. J.; Rienstra, C. M., J. Biomol. NMR. 2007, 39, 107-131. (Published online August 9, 2007)
“Chemical-shift anisotropy measurements of amide and carbonyl resonances in a microcrystalline protein with slow magic-angle spinning NMR spectroscopy”, Wylie, B. J.; Sperling, L. J.; Frericks, H. L.; Shah, G. J.; Franks, W. T.; Rienstra, C. M., J. Am. Chem. Soc. (Communication) 2007, 129, 5318-5319. (Published online April, 2007)
synuclein
“Solid-state NMR spectroscopy reveals that water is nonessential to the core structure of alpha-synuclein fibrils”, Kloepper, K. D.; Hartman, K. L.; Ladror, D. T.; Rienstra, C. M. J. Phys. Chem. B 2007, 111, 13353-13356. (Published online, November 7, 2007).
“Conformation-specific binding of alpha-synuclein to novel protein partners detected by phage display and NMR spectroscopy”, Woods, W. S.; Boettcher, J. M.; Zhou, D. H.; Kloepper, K. D.; Hartman, K. L.; Ladror, D. T.; Qi, Z.; Rienstra, C. M.; George, J. M. J. Biol. Chem. 2007, 282, 34555-34567. (Published online, September 24, 2007.).
“Temperature-dependent sensitivity enhancement of solid-state NMR spectra of alpha-synuclein fibrils”, Kloepper, K. D.; Zhou, D. H.; Li, Y.; Winter, K. A.; George, J. M.; Rienstra, C. M., J. Biomol. NMR 2007, 39, 197-211. (Published online September 28, 2007).
membrane proteins
“Chemical shift assignment of the transmembrane helices of DsbB, a 20-kDa integral membrane enzyme, by 3D magic-angle spinning NMR spectroscopy”, Li, Y.; Berthold, D. A.; Gennis, R. B.; Rienstra, C. M. Protein Sci. 2008, 17, 199-204. (Published online, February 1, 2008).
“Magic-angle spinning solid-state NMR spectroscopy of Nanodisc-embedded human CYP3A4”, Kijac, A. Z.; Li, Y.; Sligar, S. G.; Rienstra, C. M. Biochemistry 2007, 46, 13696-13703. (Published online November 7, 2007).
“Partial 13C and 15N chemical shift assignments of the disulfide bond forming enzyme DsbB by 3D magic-angle spinning NMR spectroscopy”, Li, Y.; Berthold, D. A.; Frericks, H. L.; Gennis, R. B.; Rienstra, C. M., ChemBioChem 2007, 8, 434-442. (Published online February 7, 2007).
other significant puplications
“Constant-time through-bond 13C correlation spectroscopy for assigning protein resonances with solid-state NMR spectroscopy”, Chen, L.; Olsen, R. A.; Elliot, D. W.; Boettcher, J. M.; Zhou, D. H.; Rienstra, C. M.; Mueller, L. J.; J. Am. Chem. Soc. (Communication) 2006, 128, 9992-9993. (Published online, July 19, 2006).
“Backbone conformational constraints in a microcrystalline U-15N-labeled protein by 3D dipolar-shift solid-state NMR spectroscopy”, Franks, W. T.; Wylie, B. J.; Stellfox, S. A.; Rienstra C. M., J. Am. Chem. Soc. (Communication) 2006, 128, 3154-3155. (Published online, February 16, 2006).
“Magic-angle spinning solid-state NMR spectroscopy of the ß1 immunoglobulin binding domain of protein G (GB1): 15N and 13C chemical shift assignments and conformational analysis”, Franks, W. T.; Zhou, D. H.; Wylie, B. J.; Money, B. G.; Graesser, D. T.; Frericks, H. J.; Sahota, G.; Rienstra, C. M., J. Am. Chem. Soc. 2005 127, 12291-12305. (Published online August 11, 2005).
“Site-specific 13C chemical shift anisotropy measurements in a uniformly-15N,13C-labeled microcrystalline protein by 3D magic-angle spinning NMR spectroscopy”, Wylie, B. J.; Franks, W. T.; Graesser, D. T.; Rienstra, C. M., J. Am. Chem. Soc. (Communication) 2005, 127, 11946-11947. (Published online, August 4, 2005).
“Reduction of RF-induced sample heating with a scroll coil resonator structure for solid-state NMR probes”, Stringer, J. S; Bronnimann, C. E.; Mullen, C. G.; Zhou, D. H.; Stellfox, S. A.; Li, Y.; Williams, E. H.; Rienstra, C.M. J. Magn. Reson. (Cover Article) 2005, 173, 40-48. (Published online December 15, 2004).